The PIKfyve Inhibitor YM201636 Blocks the Continuous Recycling of the Tight Junction Proteins Claudin-1 and Claudin-2 in MDCK cells
Figure 4
Claudin-4 does not accumulate following YM201636 treatment and undergoes negligible endocytosis.
(A) MDCK cells treated with YM201636 showed no detectable accumulation of internal claudin-4. Scale bars 10 µm. (B) A surface biotinylation assay was performed to measure the endocytosis of claudin-4 and a Western blot from a representative experiment is shown. (C) Results represented graphically show the mean from three independent experiments, error bars are SEM. Claudin-4 was successfully biotinylated (Surface Biotinylated) but negligible claudin-4 was internalised (Endocytosis 60 min) suggesting that claudin-4 is endocytosed at a much lower rate than claudin-1 and claudin-2.