Flip-Flop of Phospholipids in Proteoliposomes Reconstituted from Detergent Extract of Chloroplast Membranes: Kinetics and Phospholipid Specificity
Figure 8
Scramblase assay for inside labeled and outside labeled proteoliposomes.
(A) Inside labeled scramblase assay, both EGTA (control) treated (4 mM) and Ca2+ treated (2 mM) proteoliposomes showed same level of quenching. (B) Outside labeled scramblase assay, both showed same level of quenching confirming absence of scramblase activity (trace a – EGTA treated vesicles at t = 0 h, trace b - Ca2+ treated at t = 0, trace c- EGTA treated vesicles at t = 3 h, trace d – Ca2+ treated vesicles at t = 3 h).