Flip-Flop of Phospholipids in Proteoliposomes Reconstituted from Detergent Extract of Chloroplast Membranes: Kinetics and Phospholipid Specificity
Figure 6
Effect of protease (trypsin) on flippase activity from spinach chloroplast membrane proteins.
(A) Trypsin treatment before reconstitution – TE was treated with 10 mg/ml trypsin and reconstituted (trace c), trace a and b represent liposome and untreated proteoliposome (control), (p<0.001) (B) Trypsin treatment after reconstitution – Proteoliposomes were incubated with trypsin and incubated at 37°C for 30 min and flippase assay was carried out, trace a and b represent liposome and untreated proteoliposomes, trace c shows proteoliposomes treated with trypsin, (p<0.01)(C) Bar diagram shows the decrease in flippase activity when proteoliposomes were treated with varying concentrations of trypsin, (p<0.01) (D) The kinetics of flipping was altered with increase in trypsin concentration.