Type I Interferon Reaction to Viral Infection in Interferon-Competent, Immortalized Cell Lines from the African Fruit Bat Eidolon helvum
Figure 4
O'nyong-nyong virus (ONNV) replication in different mammalian cells using a high MOI.
(A) For a synchronized infection cells were inoculated with ONNV at an MOI 2.5 and supernatants were harvested at 0, 8 and 24 h post infection (hpi). After viral RNA isolation (triplicates) the concentration was measured by ONNV specific real-time RT-PCR assay. ONNV PCR units (U) per ml were determined. The dilution end-point was defined as one PCR unit. Virus replication could be detected in all cell lines. The increase of genome equivalents per ml were approximately 1000-fold after 24 hpi. (B) Titration of supernatants showed an increase of PFU per ml (titer of inoculum was subtracted) after 24 hpi of 1000 to 10000-fold. (C) The ratio of log-10 increase PFU/ml to ONNV PCR units were comparable in all cell lines indicating an efficient particle formation.