Global Mapping of H3K4me1 and H3K4me3 Reveals the Chromatin State-Based Cell Type-Specific Gene Regulation in Human Treg Cells
Figure 1
Expansion and purification of human CD4+CD25+FOXP3+ Treg cells.
Panel A, Isolation and purity (% indicated) of CD4+CD25+ T cells from human PBMCs. Panel B, Expansion rates of sorted CD4+CD25+ T cells in vitro. Panel C, Evaluation of the suppressive capacity of expanded cells. Panel D, FACS-sorting of CD4+CD25+FOXP3+ regulatory T cells and CD4+CD25+FOXP3− T cells after expansion. The purity of each sub-population is indicated. S:R, Suppressor:Responder ratios