Preventive and Therapeutic Euphol Treatment Attenuates Experimental Colitis in Mice
Figure 4
Euphol reduces pro-inflammatory cytokines and chemokines production in macrophages stimulates with lipopolysaccharide (LPS).
Macrophage from bone marrow of naïve mice were stimulated with LPS (1 µg/ml) in the presence or absence of euphol (1 and 10 µM) for 24 hours, and the culture supernatants were analyzed for cytokine levels using cytokine bead array kit (CBA). Euphol incubation in dose-related manner reduced production of MCP-1 (A), TNF-α (B), IL-6 (C), IFN-γ (D), but increase the IL-10 levels (E). Data are reported as means ± SEM (n = 4) and is representative of two independent experiments. #P<0.05 vs. control without LPS treatment (vehicle solution); *P<0.05 vs. LPS-treated group. Vehicle solution corresponds to 5% Tween 80 in medium.