Differential Sensitivity of ERBB2 Kinase Domain Mutations towards Lapatinib
Figure 6
Lapatinib-resistant ERBB2 mutants show increased transformation potential of Ba/F3 cells to cytokine independence.
(A) Ba/F3 cells transformed by ERBB2 mutants were analysed by western blotting for the activation of ERBB2 and downstream ERK phosphorylation. (B) To test the activating nature of lapatinib-resistant mutations, Ba/F3 cells were transduced with wild type or mutant MSCV-eGFP-ERBB2 and outgrowth of ERBB2-positive (green) cells with respect to parental (non-green) Ba/F3 cells was measured by FACS analysis at indicated time points.