An Inducible Cell-Cell Fusion System with Integrated Ability to Measure the Efficiency and Specificity of HIV-1 Entry Inhibitors
Figure 7
Statistical analysis of the dual reporter system with two different target cells.
A. Distribution of F-Luc and R-Luc signals obtained in the cell-cell fusion assay conducted with or without 2 µg/ml Dox. Readouts of fusion without Dox are from 35 wells of a single plate and those with Dox are from 35 wells from multiple plates. B. Statistical analysis for the precision of the system. Data from 5 (CEM-R5Luc1 cells) or 4 (Cf2Luc4#18) independent experiments were used to calculate the Z'-factor of the assay for each luciferase. Means ± standard deviations are shown for each cell type.