A Pyrene Maleimide with a Flexible Linker for Sampling of Longer Inter-Thiol Distances by Excimer Formation
Figure 2
Monomer and excimer emission in the presence of organic compounds.
A. Pyrene maleimide. B. Pyrene-4-maleimide. C. Pyrene maleimide vs. pyrene-4-maleimide in mercaptoethanol. D. Pyrene maleimide vs. pyrene-4-maleimide in butanethiol. The concentrations of the fluorescent probes and organic compounds were 3 µM and 1 mM, respectively. The labels in panels A and C also apply to panels B and D, respectively. Peaks 1–4 are indicated (see text). Data in panels A–C were normalized to peak 1 intensity in mercaptoethanol. Data in panel D were normalized to peak 4 intensity in butanethiol.