Picomolar Dichotomous Activity of Gnidimacrin Against HIV-1
Figure 4
Gnidimacrin inhibited R5 virus infection at picomolar concentrations.
(a) HIV-1 BaL and NL4-3 were used to infect CD8-depleted PHA activated PBMCs. The virus replication in the absence of antivirals is defined as 100% control virus production. Each data point in the figure represents the mean +/− standard deviation of three independent experiments. (b) Down regulation of HIV-1 receptors on PBMCs. CD8-depleted PHA activated PBMCs were treated with 1 nM of gnidimacrin or 1 uM of prostratin for 24 hr or 48 hr. Background binding was performed with the same protocol as other conditions, except that primary antibodies were not included in the assay. The color assignment to each assay condition is the same for all six FACS panels. A histogram of the FACS data on % receptor down regulation expressed as ratio of mean fluorescence intensity (MFI) is available as additional file 2 (Figure S2).