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A Mechanism for Synergy with Combined mTOR and PI3 Kinase Inhibitors

Figure 2

Baseline expression of PTEN, phospho-Akt and phospho-PDK1.

Eight endometrial cancer cell lines were grown without treatment. Total protein extracts were analyzed by Western blotting for PTEN, phospho-Akt (P-Akt S473 and P-Akt T308), total Akt, phospho-PDK1 (P-PDK1 S241), and total PDK1. β-actin expression served as a loading control.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0026343.g002