High-Throughput Screen for Identifying Small Molecules That Target Fungal Zinc Homeostasis
Figure 7
Total and labile zinc and cell morphology of C. albicans.
Candida cells treated with A, B) Atovaquone, C, D) Halofantrine or E, F) Disulfiram and incubated at 30°C for 20 hours. 100 µl aliquots were immediately used for flow cytometry, the remaining culture was used for ICP-MS analysis. Light microscopy images were obtained from cells treated with 25 µM atavaquone and halofantrine and 1 µM disulfiram using standard protocols. Error bars represent standard deviation (SD). Level of significance are characterized as followed (* P<0.01, ** P<0.001, *** P<0.0001, not significant P>0.05). Statistical significance was tested by one-way ANOVA.