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High-Throughput Screen for Identifying Small Molecules That Target Fungal Zinc Homeostasis

Figure 6

Validation of selected hits.

A subset of hit compounds was subjected to flow cytometry analysis. Cells were treated with hit compounds and incubated for 20 hours at 30°C. Labile zinc content was measured using the zinc fluorescent probe Zinbo-5. Zinbo-5 fluorescence was plotted in comparison to GFP fluorescence.

Figure 6

doi: https://doi.org/10.1371/journal.pone.0025136.g006