High-Throughput Screen for Identifying Small Molecules That Target Fungal Zinc Homeostasis
Figure 3
Dose and time response of GFP fluorescence after treatment with TPEN.
A) Cells transformed with the ZRE-GFP promoter-reporter construct were grown in RPMI-1640 medium with indicated TPEN concentrations for 20 hours at 30°C. Fluorescence signal was normalized by cell density and is expressed as x-fold increase of untreated sample. B) Cells containing the ZRE-GFP promoter-reporter construct were grown in absence or presence of 5 µM TPEN in RPMI-1640 medium at 30°C. Fluorescence and cell density were measured at times indicated. Fluorescence signal is expressed as x-fold increase of the 0 hour time point. Error bars represent standard deviation (SD).