A Stratified Transcriptomics Analysis of Polygenic Fat and Lean Mouse Adipose Tissues Identifies Novel Candidate Obesity Genes
Figure 5
RT-PCR validation of angiomodulatory genes differentially expressed in the subcutaneous adipose tissue of Fat and Lean mice.
Fat and Lean mice were fed control (FC; black bars, LC; grey bars) or high fat diet (FF; black hatched bars, LF; grey hatched bars) for 18 weeks. RNA was extracted and the gene specified above the graphs was measured by RT-PCR. Target gene expression was corrected to the expression level of the housekeeping gene tata-binding protein (TBP) and is expressed arbitrarily as an adjusted ratio. * = P<0.05 significant effect of diet within a line. † = P<0.05, ††† = P<0.001 significant difference between lines.