Newly Developed Mg2+–Selective Fluorescent Probe Enables Visualization of Mg2+ Dynamics in Mitochondria
Figure 5
KMG-301 was not released from mitochondria upon depolarization of their membrane potential.
(A) FCCP induced a decrease in fluorescence of rhodamine 123 in the cell body (presence of mitochondria, gray line). On the other hand, in the nucleus area (no mitochondria area), an increase in fluorescence intensity was observed (black line). It is caused by the release of the probe from mitochondria to the cytosol. These lines show the time-course average of 60 cells. (B) Whereas FCCP also induced a decrease in fluorescence of KMG-301 in the cell body (gray line), no increase in fluorescence was observed in the nucleus area (no mitochondria area, black line). This data indicates that KMG-301 is not released from mitochondria depending on the depolarization of their membrane potential. These lines show the time-course average of 43 cells. (C) Fluorescence images and pseudo-colored images (F/F0) of non-differentiated PC12 cells stained with Rh123 and KMG-301. Upon FCCP administration, increases in fluorescence in the nucleus area were observed only in Rh123 loaded cells. Red circles indicate the nucleus areas. The scale bar indicates 10 µm.