Newly Developed Mg2+–Selective Fluorescent Probe Enables Visualization of Mg2+ Dynamics in Mitochondria
Figure 4
KMG-301 is functional in mitochondria in living cells.
(A) Time-course of the changes in the fluorescence of KMG-301 in PC12 cells expressing negative control miR RNAi (gray line) and miR RNAi for Mrs2 (black line). An increase in the extracellular Mg2+ concentration from 0 mM to 10 mM induced an increase in the fluorescence of KMG-301 in PC12 cells expressing negative control miR RNAi. This increase in fluorescence was significantly suppressed in PC12 cells expressing miR RNAi for Mrs2. (B) Comparison of the relative fluorescence intensities of KMG-301 1 minute after the increase in extracellular Mg2+ concentration. Both two miR RNAis significantly inhibited the increase in fluorescence of KMG-301. This data indicates that KMG-301 was internalized in mitochondria and responded to changes in [Mg2+]mito. The error bars indicate SEM. * indicates p<0.05, and ** indicates p<0.01.