Newly Developed Mg2+–Selective Fluorescent Probe Enables Visualization of Mg2+ Dynamics in Mitochondria
Figure 3
KMG-301 detects the changes in Mg2+ concentration in isolated mitochondria.
(A) Time-course and pseudo-colored images of the change in the fluorescence of KMG-301 in isolated mitochondria stained with KMG-301AM. Stepwise increases in the extramitochondrial Mg2+ concentration induced increases in the fluorescence of KMG-301 in mitochondria. This indicates that Mg2+ absorption in mitochondria was measured successfully with KMG-301. The scale bar indicates 10 µm. (B) Time-course and pseudo-colored images of the change in the fluorescence of KMG-301 in response to 5 µM FCCP. The FCCP–induced release of Mg2+ from mitochondria was observed successfully. (C) Mean fluorescence intensities of TMRE before stimuli, 5 min after FCCP treatment and 10 min after washout in isolated mitochondria from PC12 cells. The membrane potential partially recovered after washout of FCCP. (D) Mean fluorescence intensities of KMG-301 before stimuli, 5 min after FCCP treatment and 10 min after washout. Fluorescence of KMG-301 was increased upon repolarization of the mitochondrial membrane potential. This data indicates that the fluorescence of KMG-301 is responding to the changes in [Mg2+]mito. The error bars indicate SEM.