Lucanthone and Its Derivative Hycanthone Inhibit Apurinic Endonuclease-1 (APE1) by Direct Protein Binding
Figure 13
Lucanthone does not alter conformation of double hydrophobic site APE1 mutant, human Nth and bacterial Nfo proteins.
CD spectra of APE1 and its mutants in presence of lucanthone. APE1- hydrophobic site mutant proteins F266A/C, F266A/W280A, active site mutant D210N and non-related BSA protein or human Nth or E.coli Nfo(10 mg/ml), 50 µl (500 µg) (14 µM) in APE1 buffer (50 mM HEPES, 150 mM KCl, 5 mM MgCl2), was mixed with lucanthone (1 mg/ml), 50 µl (50 µg) (140 µM), incubated at 37°C for 60 min and far UV-CD spectra with specifications.