Lucanthone and Its Derivative Hycanthone Inhibit Apurinic Endonuclease-1 (APE1) by Direct Protein Binding
Figure 9
Identification of an approximate lucanthone cleavage site in APE1.
LC/MS/MS identification of APE1 fragment after treatment with 100 µM of lucanthone for 2 h at 37°C. The data were analyzed with Inspect. A). Peptide aa64–73 analysis of 35.5 Kda (Sample 1) and 25 kDa (Sample 3). B). Elution profile of peptide aa64–73 in sample 1 and 3. C). Peptide aa282–299 analysis of 35.5 Kda (Sample 1) and 25 kDa (Sample 3). D). Peptide aa53–63 analysis of 35.5 Kda (Sample 1) and 25 kDa (Sample 3). B). Elution profile of peptide aa53–63 in sample 1 and 3.