Lucanthone and Its Derivative Hycanthone Inhibit Apurinic Endonuclease-1 (APE1) by Direct Protein Binding
Figure 6
Thioxanthenones and CRT cleavage of APE1 is inhibited by Ascorbic acid but not by N-Acetyl cysteine.
Western blot of recombinant APE1 protein (250 ng) treated with 100 µM of radical quenchers, NAC and Ascorbic acid and 100 µM of lucanthone (L)/hycanthone (H) or 200 µM of CRT (C) at 37°C for 2 h.