Lucanthone and Its Derivative Hycanthone Inhibit Apurinic Endonuclease-1 (APE1) by Direct Protein Binding
Figure 5
Lucanthone promotes APE1 cleavage in vitro which is inhibited by TRIS.
Western blot of recombinant APE1 protein (250 ng) treated with 10–50 µM lucanthone at 37°C for 2 h in absence and presence of radical quencher, 10 mM Tris-HCl, pH 7.4.