Lucanthone and Its Derivative Hycanthone Inhibit Apurinic Endonuclease-1 (APE1) by Direct Protein Binding
Figure 1
Lucanthone/Hycanthone promotes APE1 cleavage in presence of CHX and this cleavage is inhibited by 1% DMSO.
Western blot of total cell extract from APE1-5 overexpresser clone pretreated with 10 µg/ml of cycloheximide for 4 h followed by 25–100 µM lucanthone/hycanthone for 2 h (12.5 mg of total cell protein loaded per lane).