Anti-Angiogenic and Anti-Inflammatory Properties of Kahweol, a Coffee Diterpene
Figure 9
Kahweol inhibits HUVEC endothelial cell COX-2 expression and MCP-1 secretion in a dose-dependent manner.
A) Typical results of a Western blot assay using anti-COX-2 antibodies. B) Quantification of the normalized relative inhibitory effect. Data are given as percentage, taking the normalized levels of COX-2 in control cells as 100%, and they are means±S.D. of three different assays. C) Quantification of the amount of MCP-1 secreted by HUVEC after a 24 h treatment in the presence of different concentrations of kahweol. Data are given as femtograms of secreted MCP-1 per cell, and they are means±S.D. of three different experiments. *Statistically significant (p<0.01) as compared to control values, according to a two-tailed Student's t-test.