Microfluidic Perfusion for Regulating Diffusible Signaling in Stem Cells
Figure 5
Graphical model of signaling in mESC neuroectodermal differentiation.
Removing cell-secreted factors suppresses growth and differentiation, which can be restored by supplementing N2B27 medium with cell-secreted factors. Supplementation with (cell-secreted) FGF4 does not rescue growth of perfused mESC cultures, while inhibition of FGF signaling downregulates differentiation without compromising mESC growth.