Prospective Genomic Characterization of the German Enterohemorrhagic Escherichia coli O104:H4 Outbreak by Rapid Next Generation Sequencing Technology
Figure 5
Evolutionary model of the origin of the German EHEC O104:H4 outbreak strain.
Evolutionary model of the current outbreak strain (LB226692) and the historical strain (01-09591) from 2001 based on whole chromosomal and plasmid data. Numbers on connecting lines indicate the number of loci that differ between the strains as determined by analysis of 1,144 core genome genes. The genes of the PCR test for differentiation of the HUSEC041 complex (stx2: red; terD: blue; rfbO104: green; fliCH4: yellow), [16] for antibiotic resistance (orange: TEM-1 and CTX-M-15), and for the differentiation of EAEC plasmids [5] (astA: white) are colored. The order of plasmid acquisition and loss were arbitrarily chosen in the illustration as the exact sequence of events is not known. Year of isolation for each strain is noted in the lower left corner of each rectangle.