Integrated Expression Profiling and Genome-Wide Analysis of ChREBP Targets Reveals the Dual Role for ChREBP in Glucose-Regulated Gene Expression
Figure 2
Characterization of ChREBP binding sites at selected gene loci.
(A, B) CisGenome Browser screenshots of peaks associated with the PKLR and TXNIP genes. The y-axis shows the number of mapped tags. Annotations are from the UCSC Genome Browser. (C) Seven ChREBP binding sites in six target genes showing peak height and fold enrichment. ChIP-qPCR was performed to confirm the identified ChREBP binding sites. The fold enrichment is the fold increase for the signal from ChREBP ChIPed DNA relative to control IgG. Cyclophilin (Cyclo) and PKLR-4 kb were used as negative controls (0.98- and 1.2-fold enrichment, respectively).