Histone Deacetylase Inhibition Enhances Self Renewal and Cardioprotection by Human Cord Blood-Derived CD34+ Cells
Figure 8
Effect of VPA and CTR cells on myocardial healing.
(A) Representative images of α-SMA staining (red fluorescence) of the infarct zone to reveal the presence of myo-fibroblasts. (B) Representative low and high power views of picrosirius red staining of the myocardium to reveal the collagen deposition. Pictures on the right show polarized light imaging of the same microscopic fields in the center of the panel, to show that birefringence of collagen bundles was not affected by saline or CTR and VPA CD34+ cells treatments. (C–D) Quantification of Collagen deposition and myofibroblasts. Collagen data are shown as percentage of the areas containing collagen normalized to total area sections, while myofibroblasts were determined by counting the number of α-SMA+ cells in the infarct zone (n≥6). Statistical analysis of these data by one-way ANOVA with Newman-Keuls post-hoc test did not reveal differences between treatment groups, although lower amount of Collagen and smaller myofibroblasts number were found in VPA cells-injected mice.