Histone Deacetylase Inhibition Enhances Self Renewal and Cardioprotection by Human Cord Blood-Derived CD34+ Cells
Figure 7
Enhanced cytokine release and higher hypoxia suppression by 7 days VPA-treated CD34+ cells conditioned medium.
(A) Analysis of pro-inflammatory and pro-angiogenic factors present in the control and VPA-treated CD34+ cells secretome. Table shows means and standard error of each cytokine released in the culture supernatants. Heat maps indicate a treatment-related coherent upregulation of most of the secreted factors in four independent CD34+ cells samples. (B) Statistical analysis of the cytokine concentrations in culture supernatants by paired t-test. The fold change in the release of these cytokines from VPA-treated vs. CTR CD34+ cells is shown. With the exception of Leptin, IL-8, VEGF, and bFGF (orange color), all the other cytokines were significantly up-regulated in HDACi preconditioned cells, indicating an enhancement of their paracrine effect. (C) Effect of CTR and VPA CD34+ cells conditioned medium on rescue from apoptosis of HL-1 cardiomyocytes cell line exposed to hypoxia conditions. Data in the graph represent the percent variation in apoptotic death of HL-1 cells exposed to hypoxia in the presence of VPA treated CD34+ cells conditioned medium in comparison with medium conditioned by CTR cells; * indicate P<0.05 by paired t-test (n = 5).