Histone Deacetylase Inhibition Enhances Self Renewal and Cardioprotection by Human Cord Blood-Derived CD34+ Cells
Figure 6
Effect of 7 days cultured CTR and VPA-treated CD34+ cells on survival, left ventricle function neo-vascularization and ventricular remodeling in an immunodeficient mouse model of myocardial infarction.
(A) Mortality Kaplan-Meier curve of sham operated, saline-injected, control and VPA CD34+ cells-injected mice. The mortality in mice injected with VPA-treated CD34+ cells was not significantly different from that of sham operated mice (see enclosed table). Significance calculated by log-rank (Mantel-Cox) test. (B) Echocardiographic assessment of ejection fraction, left ventricular end-diastolic and end-systolic volumes showed an improved ventricular function in VPA treated CD34+ cells, but not in control CD34+ cells-injected animals. (C) Representative images of transversal sections of diastole-arrested hearts, used to evaluate the infarct scar size and LV morphometric values. * indicate P<0.05 by one way ANOVA with Newman Keuls post hoc analysis (n≥7). (D) Representative images of Rhodamine-labeled Griffonia simplicifolia Lectin 1 staining of LV histological sections for capillary density determination. The bar graph indicates the density of these vessels at the infarct border zone.