Histone Deacetylase Inhibition Enhances Self Renewal and Cardioprotection by Human Cord Blood-Derived CD34+ Cells
Figure 3
Effect of VPA is directly related to HDAC inhibition.
(A) The VPA structural analogue Valpromide (VPM) reduced CD34+ cells proliferation at lower extent compared to continuous treatment with VPA in a 5 day time course experiment. By contrast, the CD34 expression profile was identical to that of control cells, as detected by flow cytometry. (B) Western blotting showing that a 7 day VPA treatment induced hyper acethylation on H4 and H3 (at lysine residue 9) histones. * indicate P<0.05 by two ways ANOVA with Bonferroni post hoc analysis (n≥3). (C) Representative ChIP experiment showing an increased enrichment of various sites in the CD34 gene promoter (upstream and downstream of the TSS), as a result of chromatin hyper-acetylation (pan-H4Ac, H3K9Ac) due to VPA treatment. Data are expressed as relative enrichment calculated by real time PCR amplification.