CD103 Deficiency Prevents Graft-versus-Host Disease but Spares Graft-versus-Tumor Effects Mediated by Alloreactive CD8 T Cells
Figure 5
CD103 is not required for accumulation of CD8 T cells in the lamina propria or mucosal lymphoid compartments during GVHD.
Equal numbers of CD8 T cells from BALB/c-WT (WT, Thy1.1+) and BALB/c-CD103 KO (KO, Thy1.1+Thy1.2+) donors were adoptively transferred into a group of lethally irradiated A/J mice together with BALB/c-WT BMC plus SaI/N tumor cells. Recipient mice were sacrificed at day 21. Lymphocytes isolated from the indicated host compartments were subjected to flow cytometric analyses. A. Representative dot plots of WT and KO CD8 T cells in the host intestinal epithelium (IEL), lamina propria (LPL), Peyer's patch (PPL), and mesenteric lymph node (MLN) at d21 post alloSCT. B. Mean ratios ± SEM (n = 5) of WT to KO CD8 T cells in the indicated compartments. *p<0.05 compared with IEL; **p-value<0.01 compared with IEL.