Suppression of STAT3 and HIF-1 Alpha Mediates Anti-Angiogenic Activity of Betulinic Acid in Hypoxic PC-3 Prostate Cancer Cells
Figure 3
Effect of betulinic acid (BA) on hypoxia-induced STAT3 activation in PC-3 cells.
PC-3 cells were treated with or without BA (5 or 10 µM) under normoxic or hypoxic condition for 4 h. (A) Cell lysates were prepared and subjected to Western blotting for phospho-STAT3 and STAT3. (B) Nuclear extracts were prepared and applied to EMSA to analyze the STAT3-DNA binding activity. (C) Cells were treated with or without BA (10 µM) under hypoxia. Immunocytochemistry was performed for STAT3. DAB (brown) and hematoxylin-eosin was used as a substrate and a counterstaining, respectively.