DNA-PK-Dependent RPA2 Hyperphosphorylation Facilitates DNA Repair and Suppresses Sister Chromatid Exchange
Figure 4
S4, S8 phosphorylation in RPA2 delays mitotic entry and confers resistance to 4NQO.
(A) Scheme for HU treatment. (B) RPA2 S4A S8A cells enter mitosis more frequently after release from HU treatment. More than 50,000 cells were counted for each cell type. The average from three independent experiments with standard deviation is presented. (C) Silencing the expression of DNA-PKcs makes cells enter mitosis more frequently after release from HU treatment. More than 50,000 cells were counted for each cell type. The average from three independent experiments with standard deviation is reported. (D) Cells expressing the RPA2 S4A S8A mutant are more sensitive to 4NQO.