Functional Stability of Unliganded Envelope Glycoprotein Spikes among Isolates of Human Immunodeficiency Virus Type 1 (HIV-1)
Figure 6
Time course dissociation of HIV-1 Env trimers in the presence of membrane altering reagents visualized using BN-PAGE.
HIV-1LAI-JR-FL MC, produced in 293T cells, was incubated for 0 h, 4 h, 8 h, 24 h, or 96 h at 37°C in the presence of no compound (left), 70 mM β-cyclodextrin (cholesterol scavenger; center), or 1% DDM (mild detergent; right). Following incubation for the indicated time periods, samples were analyzed by BN-PAGE and Western blot as in Fig. 4.