Functional Stability of Unliganded Envelope Glycoprotein Spikes among Isolates of Human Immunodeficiency Virus Type 1 (HIV-1)
Figure 5
Time course incubation of HIV-1 Env trimers at physiological temperature (37°C) visualized using BN-PAGE.
(A) HIV-1LAI-JR2 MC, produced in 293T cells, was incubated for various time intervals up to 96 h at 37°C and aliquots removed for BN-PAGE and Western blot analysis, as in Fig. 4. Down arrows indicate the time interval in which infectivity of the cognate virus decreases by ≥90%. The band smearing in the 72 h lane is an experimental artifact of sample loading. (B) The same virus sample as in Panel A was subjected to indicated temperatures for 1 h and analyzed on BN-PAGE, as in Fig. 4, except that the electrophoresis run time was shorter causing less separation between different bands.