A Novel Anti-CEACAM5 Monoclonal Antibody, CC4, Suppresses Colorectal Tumor Growth and Enhances NK Cells-Mediated Tumor Immunity
Figure 6
mAb CC4 blocked the suppression of NK killings induced by over-expression of CEACAM5 in colorectal cancer cells.
Forced expressions of CEACAM1 and CEACAM5 in HCT-15 cells were confirmed by flow cytometry (A). Stable transfectants were then subjected to NK cytotoxicity assays using CD56+CD16+ NK cells (B) and CD56+CD16- NK cells under different ratios of numbers of target cells to that of effector cells (C and D). Significant differences between mAb CC4 treated or RACEA-treated groups and the corresponding control groups were indicated by double stars (**P<0.01).