A Novel Anti-CEACAM5 Monoclonal Antibody, CC4, Suppresses Colorectal Tumor Growth and Enhances NK Cells-Mediated Tumor Immunity
Figure 2
mAb CC4 was able to target xenografted tumors, suppress tumor growth in vivo, and raised ADCC reactions in vitro.
Non-small cell lung cancer cells A549 was stained with cy5 labeled CC4 in immunofluorescence (A). Cells were injected to nude mice to form tumors and CC4-cy5 or IgG-cy5 was administrated intravenously. In vivo optical imaging of fluorescent antibodies was performed in the indicated times (B and C). LS174T cells were injected subcutaneously into nude mice. After forming tumor, mice were treated with CC4 or normal IgG control for indicated times. Tumors were taken out for measuring the size and calculating the volume, as well as photography (D, n = 7 in each group, **P<0.01 and *P<0.05). LS174T cells were subjected to ADCC assays using murine spleen cells as effector cells. Results for specific cytotoxicity of cancer cells were presented in bar graphs (E).