The Slow-Releasing Hydrogen Sulfide Donor, GYY4137, Exhibits Novel Anti-Cancer Effects In Vitro and In Vivo
Figure 1
Differential H2S-releasing manner of NaHS and GYY4137.
(A) H2S-releasing profile of NaHS, GYY4137 and ZYJ1122. H2S released from NaHS, and GYY4137 (400 µM) was determined in aliquots (100 µl) of medium withdrawn at timed intervals (up to 7 days) from cultured MCF-7 cells. Concentration of H2S amounts was assessed spectrophotometrically using N,N-dimethyl-p-phenylenediamine-dihydrochloride and results showed the H2S concentration (µM). H2S release from GYY4137 was significantly different (P<0.05) from T = 0 at all time points from 0.3 hour to 7 days. H2S release from NaHS was significantly different (P<0.05) from T = 0 at all time points up to 1.5 hours. No detectable H2S was released from ZYJ1122 (400 µM) under identical experimental conditons. Results show mean ± s.e. mean, n = 3. The chemical structures of GYY4137 and ZYJ1122 are shown in the inset. (B) Growth curve analyses of MCF-7, HL-60 and MV4-11 cells treated with NaHS, GYY4137 and ZYJ1122 (400 µM) over 5 days. Cell survival was determined by trypan blue staining. Results show cell growth as a percentage relative to NT cell numbers at day 5 and are mean ± s.e. mean, n = 3.