Evaluation of Cell Cycle Arrest in Estrogen Responsive MCF-7 Breast Cancer Cells: Pitfalls of the MTS Assay
Figure 1
Comparative analysis of MCF-7 cell viability, cell number and mitochondrial activity.
Cells were treated with 5 mM IPTG, 10 nM ICI 182780, 5 µM FTY720, or serum deprived (serum free) 48 h post-seeding. Using the Trypan Blue exclusion method cells were harvested and viable cells counted using a haemocytometer at days indicated. MTS and SYBR-DNA assays were performed, as detailed in materials and methods, at days indicated. The treatment results are shown as a percentage of the uninduced vehicle control (±SE) correlating with viable cell number (Trypan blue counts), colorimetric measurement (MTS), and fluorescent intensity (SYBR assay). Each experiment was performed in triplicate at least 3 times with similar results.