Study of Histopathological and Molecular Changes of Rat Kidney under Simulated Weightlessness and Resistance Training Protective Effect
Figure 5
Immunohistochemical staining and real-time PCR analysis of apoptosis.
A: Histological sections of kidneys in CON, TS and TS&RT groups (A1–A3) respectively were stained with anti Bax protein serum. B: Histological sections of kidneys in CON, TS and TS&RT groups (B1–B3) respectively were stained with anti Bcl-2 protein serum. C: Histological sections of kidneys in CON, TS and TS&RT groups (C1–C3) respectively were stained with anti caspase-3 protein serum. D: The protein ratio of Bax/Bcl-2 is obtained by dividing signal intensity of Bax positive substance and Bcl-2 positive substance. E: Semi-quantitative analysis of caspase-3 protein with immunohistochemical staining. F and G: mRNA ratio of Bax/Bcl2 and relative mRNA levels of caspase-3 in CON, TS and TS&RT rats' kidney A total of 10 samples for each group were used, and each sample was run in triplicate for real-time PCR. Data are shown as means±SD. *P significant p values<0.05 and **P significant p values<0.01.