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Study of Histopathological and Molecular Changes of Rat Kidney under Simulated Weightlessness and Resistance Training Protective Effect

Figure 2

Histopathological analysis of rats' kidneys paraffin sections stained with hematoxylin and eosin (HE), mallory's trichrome staining and sirius red staining.

A: Morphological changes of renal cortex: The normal structure of rats' kidney in the control group (A1). In TS group, rat's renal cortex showed glomerular atrophy, distension of the renal glomerulus capsular space (arrowhead) and interstitial edema (arrow, A2). In TS&RT group, rat's renal cortex displayed moderately glomerular atrophy (arrowhead) and interstitial congestion (arrow, A3). B: Morphological changes of renal medulla: The normal structure of rats' kidney in the control group (B1). In TS group, rat's renal medulla abundantly illustrated degeneration and necrosis of renal tubular epithelial cells (arrowhead), interstitial congestion (arrow), and protein and cellular cast in nephric tubules (B2). In TS&RT group, rat's renal medulla showed scattered degeneration and necrosis of renal tubular epithelial cells (arrowhead, B3). C&D: Mallory's trichrome (C) and Sirius Red (D) stained paraffin sections displayed different levels of proliferation of kidney fibrous tissue in CON(C1,D1), TS(C2,D2) and TS&RT groups (C3,D3)respectively. Collagen fibers were stained blue with Mallory's trichrome and red with Sirius Red respectively (arrow).

Figure 2

doi: https://doi.org/10.1371/journal.pone.0020008.g002