Sour Taste Responses in Mice Lacking PKD Channels
Figure 2
Expression of PKDs in taste tissues.
A. mRNA expression of PKD1L3 and PKD2L1 in taste tissues of WT, PKD1L3−/−, PKD2L1−/−, PKD1L3/2L1dbl−/−, GAD-GFP, GAD-GFP+PKD1L3−/−, and GAD-GFP+PKD2L1−/− mouse. FP: fungiform taste buds. CV: circumvallate taste buds. ET: epithelial tissue. Gustducin is a control for taste tissue. β-actin is an internal control. 100 bp marker was used. B, C. Immunostaining for PKD1L3 (B) and PKD2L1 (C) in taste tissues of GAD-GFP+WT mouse. D. Immunostaining for PKD1L3 in taste tissues of GAD-GFP+PKD1L3−/− mouse. E. Immunostaining for PKD2L1 in taste tissues of GAD-GFP+PKD2L1−/− mouse. F. Immunostaining for GAD67 in taste tissue of GAD-GFP+WT mouse. G. Immunostaining without primary antibody in GAD-GFP+WT mouse. Green shows GFP fluorescence. Red shows immunoreactivity (IR) for PKD1L3, PKD2L1 or GAD67, respectively. FP: fungiform papillae. CV: circumvallate papillae. Scale bar, 10 µm.