Crystal Structure of the PAC1R Extracellular Domain Unifies a Consensus Fold for Hormone Recognition by Class B G-Protein Coupled Receptors
Figure 6
Stimulation of cAMP signaling by PACAP38-MBP.
AD293 cells transiently transfected with PAC1R were stimulated with PACAP38-MBP for 4 hrs at 37°C after which the cells were lysed and assayed for cAMP content. All data points are average of duplicate samples and normalized to the stimulation level of wild-type receptor and peptide. (a) 0.03 nM PACAP38-MBP mutants were used to stimulate AD293 cells transiently expressing full length PAC1R-wt. (b) 0.03 nM PACAP38-MBP wt was used to stimulate cells transiently expressing PAC1R mutants.