A New Role for an Old Antimicrobial: Lysozyme c-1 Can Function to Protect Malaria Parasites in Anopheles Mosquitoes
Figure 3
Lysozyme c-1 knockdown (KD) significantly reduced prevalence and intensity of P. berghei infections.
The results of three independent replicate experiments for the An. gambiae G3 strain are shown. (A) Shows the significantly decreased prevalence of infection in the LYSC-1 KD mosquitoes. Prevalence was defined as the percentage of mosquitoes that had at least one oocyst per mosquito midgut. (B) Oocyst count data from the three independent replicate experiments including zeros or excluding zeros (mosquitoes that had no infection) is presented. A two-way ANOVA was conducted on individual experiments and p-values are presented along with mean ± SE of oocysts counted in each experiment. The p-value for the totals row indicates the significance of the test when the effect of experiment was included. Parasite numbers were significantly lower in the LYSC-1 KD mosquitoes as compared to control GFP group. ¶Indicates the fold reduction in the expression LYSC-1 as compared to control GFP groups as determined via densitometry analysis (see Materials and Methods).