Rational Design of Protein Stability: Effect of (2S,4R)-4-Fluoroproline on the Stability and Folding Pathway of Ubiquitin
Figure 3
Profiles of melting temperature against pH and unfolding enthalpy against melting temperature.
(A) Dependence of denaturation temperature Tm on pH for wt-ub (black circles) and (4R)-FPro-ub (red circles). (B) Unfolding enthalpy versus denaturation temperature for wt-ub (black circles) and (4R)-FPro-ub (red circles).