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Rational Design of Protein Stability: Effect of (2S,4R)-4-Fluoroproline on the Stability and Folding Pathway of Ubiquitin

Figure 2

ESI-MS analyses.

ESI-MS analyses of wt-ub (A), (4R)-FPro-ub (B). The minor peak corresponds to the double substituted protein. (C) wt-ub and (4R)-FPro-ub after purification on 15% SDS-PAGE.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0019425.g002