Rational Design of Protein Stability: Effect of (2S,4R)-4-Fluoroproline on the Stability and Folding Pathway of Ubiquitin
Figure 1
Tertiary structure of human ubiquitin according to the 1.8 Å crystal structure (1UBQ.pdb).
All three proline residues (shown in red) display the Cγ-exo conformation and were simultaneously substituted with (2S,4R)-4-fluoroproline ((4R)-FPro-ub).