The AP-1 Binding Sites Located in the pol Gene Intragenic Regulatory Region of HIV-1 Are Important for Viral Replication
Figure 10
The splicing pattern of HIV-1 transcripts is unaffected by the mutations introduced in the intragenic AP-1 binding sites.
Total RNA was extracted from infected Jurkat or U937 cells (with wild-type or mutant virus stocks) at 5 days post-infection. After DNase I treatment, total RNA was reverse-transcribed with random primers and first strand cDNAs were quantified with primers designed to quantify full-length unspliced viral mRNAs, singly-spliced viral mRNAs and multiply-spliced viral mRNAs. The total amount of viral mRNAs for each virus was arbitrarily attributed a value of 100% and the proportion of each type of transcript is presented as histograms indicating the means and standard errors of the means from two independent experiments performed in duplicate.