The AP-1 Binding Sites Located in the pol Gene Intragenic Regulatory Region of HIV-1 Are Important for Viral Replication
Figure 3
Identification of point mutations which abolish AP-1 transcription factors' in vitro binding to their respective binding sites located in fragment 5103.
(A) The wild-type and mutated AP-1#1, AP-1#2 and AP-1#3 oligonucleotide sequences are indicated as well as the corresponding underlying amino acid sequence of the viral reverse transcriptase. Base pairs which were modified in the mutated versions of the AP-1 binding sites relative to the wild-type versions are indicated by asterisks. (B) The AP-1#1, AP-1#2 and AP-1#3 oligonucleotide probes were incubated with nuclear extracts (10 µg) from mock-treated (lane 1) or PMA-treated (lanes 2 to 6) HeLa cells in the absence of competitor (lanes 1 and 2) or in the presence of increasing molar excesses (2 and 4 fold) of each respective homologous AP-1 oligonucleotide (lanes 3 and 4) or of the corresponding mutated AP-1 oligonucleotide (lanes 5 and 6). The figure shows the specific retarded bands of interest, which are indicated by arrows.